NuMA Polyklonaler Antikörper

NuMA Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 16607-1-AP

Synonyme

NUMA1, NMP22, NMP-22, Nuclear matrix protein-22, Nuclear mitotic apparatus protein

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  WB analysis using 16607-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16607-1-AP (NuMA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 16607-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16607-1-AP (NuMA antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 16607-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16607-1-AP (NuMA antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HCT 116 using 16607-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using NuMA antibody (16607-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, COS-7-Zellen, K-562-Zellen, NIH/3T3-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HCT 116-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

16607-1-AP bindet in WB, IHC, IF/ICC, ELISA NuMA und zeigt Reaktivität mit Affe, human, Maus, Ratten

• Getestete Reaktivität: Affe, human, Maus, Ratte
• In Publikationen genannte Reaktivität: Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NuMA fusion protein Ag9815
• Vollständiger Name: nuclear mitotic apparatus protein 1
• Berechnetes Molekulargewicht: 238 kDa
• Beobachtetes Molekulargewicht: 238 kDa
• GenBank-Zugangsnummer: BC013023
• Gene symbol: NuMA
• Gene ID (NCBI): 4926
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NUMA1, also named as Nuclear matrix protein-22, is a 2115 amino acid protein, which forms multiarm oligomers by association of C-terminal tail domains. NUMA1 resides in the nuclear matrix during interphase. NUMA1 is a highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role and occupies the majority if the nuclear volume. NUMA1 is required for maintenance and establishment of the mitotic spindle poles during symmetric cell divisions, functioning as a tether linking bulk microtubules of the spindle to centrosomes.

Publikationen

Species	Application	Title
pig	IF	J Cell Sci; RNA localization to the mitotic spindle is essential for early development and is regulated by kinesin-1 and dynein; Authors - Carolyn M Remsburg