NUMB Rekombinanter Antikörper

NUMB Rekombinant Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250417F6

Kat-Nr. : 85939-4-PBS

Synonyme

C14orf41, h Numb, h-Numb

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  WB analysis using 85939-4-RR (same clone as 85939-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85939-4-RR (NUMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85939-4-PBS in a different storage buffer formulation.

• 

  WB analysis using 85939-4-RR (same clone as 85939-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85939-4-RR (NUMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85939-4-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 85939-4-RR (same clone as 85939-4-PBS)

  IP result of anti-NUMB (IP:85939-4-RR, 4ug; Detection:85939-4-RR 1:3000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 85939-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 85939-4-RR (same clone as 85939-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85939-4-RR (NUMB antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85939-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 85939-4-RR (same clone as 85939-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85939-4-RR (NUMB antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85939-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 85939-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85939-4-RR against Human NUMB were performed. The affinity constant is 1.15 nM.

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Geprüfte Anwendungen

Produktinformation

85939-4-PBS bindet in WB, IHC, IP, Indirect ELISA NUMB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NUMB fusion protein Ag31553
• Vollständiger Name: numb homolog (Drosophila)
• Berechnetes Molekulargewicht: 71KD
• Beobachtetes Molekulargewicht: 66-71 kDa
• GenBank-Zugangsnummer: NM_001005743
• Gene symbol: NUMB
• Gene ID (NCBI): 8650
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NUMB, also named as S171, plays a role in the determination of cell fates during development. It is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The degradation of NUMB is induced in a proteasome-dependent manner by MDM2. Four transcript variants encoding different isoforms have been found for this gene.