NUMB Rekombinanter Antikörper

NUMB Rekombinant Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

250417F6

Kat-Nr. : 85939-4-RR

Synonyme

C14orf41, h Numb, h-Numb

---

• 

  WB analysis using 85939-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85939-4-RR (NUMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85939-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85939-4-RR (NUMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 85939-4-RR

  IP result of anti-NUMB (IP:85939-4-RR, 4ug; Detection:85939-4-RR 1:3000) with HeLa cells lysate 1600 ug.

• 

  IHC staining of human breast cancer using 85939-4-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85939-4-RR (NUMB antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 85939-4-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85939-4-RR (NUMB antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 85939-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85939-4-RR against Human NUMB were performed. The affinity constant is 1.15 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, Maushirngewebe, NIH/3T3-Zellen, Rattenhirngewebe, SKOV-3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85939-4-RR bindet in WB, IHC, IP, ELISA NUMB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NUMB fusion protein Ag31553
• Vollständiger Name: numb homolog (Drosophila)
• Berechnetes Molekulargewicht: 71KD
• Beobachtetes Molekulargewicht: 66-71 kDa
• GenBank-Zugangsnummer: NM_001005743
• Gene symbol: NUMB
• Gene ID (NCBI): 8650
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NUMB, also named as S171, plays a role in the determination of cell fates during development. It is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The degradation of NUMB is induced in a proteasome-dependent manner by MDM2. Four transcript variants encoding different isoforms have been found for this gene.