NUMBL Monoklonaler Antikörper

NUMBL Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5F6E1

Kat-Nr. : 66155-1-PBS

Synonyme

CAG3A, CTG3a, NBL, numb homolog (Drosophila) like, Numb like protein, NUMB R, Numb related protein, NUMBL, NUMBLIKE, NUMBR, TNRC23

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  WB analysis of L02 using 66155-1-Ig (same clone as 66155-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66155-1-Ig (NUMBL antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66155-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66155-1-Ig (same clone as 66155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66155-1-Ig (NUMBL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66155-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66155-1-Ig (same clone as 66155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66155-1-Ig (NUMBL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66155-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66155-1-Ig (same clone as 66155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66155-1-Ig (NUMBL antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66155-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66155-1-Ig (same clone as 66155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66155-1-Ig (NUMBL antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66155-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-12 using 66155-1-Ig (same clone as 66155-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using 66155-1-Ig (NUMBL antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66155-1-PBS in a different storage buffer formulation.

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  IF Staining of L02 using 66155-1-Ig (same clone as 66155-1-PBS)

  Immunofluorescent analysis of L02 cells using 66155-1-Ig (NUMBL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66155-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66155-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA NUMBL und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NUMBL fusion protein Ag16771
• Vollständiger Name: numb homolog (Drosophila)-like
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC001794
• Gene symbol: NUMBL
• Gene ID (NCBI): 9253
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Numbl (also known as numblike or Nbl), as a conserved homolog of Drosophila Numb, is specfically expressed in the brain. Numbl is a cytoplasm protein and has redundant functions in embryonic neurogenesis. It plays an important role in ependymal wall integrity and subventricular zone neuroblast survival. However, its expression and function in the central nervous system lesion are still unclear.