NUP107 Polyklonaler Antikörper

NUP107 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Rezensionen (1)

Kat-Nr. : 19217-1-AP

Synonyme

107 kDa nucleoporin, nucleoporin 107kDa, Nucleoporin Nup107, NUP107, NUP84

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Galerie der Validierungsdaten

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  WB analysis using 19217-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19217-1-AP (NUP107 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 19217-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19217-1-AP (NUP107 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 19217-1-AP

  IP Result of anti-NUP107 (IP:19217-1-AP, 4ug; Detection:19217-1-AP 1:1000) with HeLa cells lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HeLa-Zellen, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

19217-1-AP bindet in WB, IP, IHC, IF, ELISA NUP107 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NUP107 fusion protein Ag5250
• Vollständiger Name: nucleoporin 107kDa
• Berechnetes Molekulargewicht: 106 kDa
• Beobachtetes Molekulargewicht: 107 kDa
• GenBank-Zugangsnummer: BC017167
• Gene symbol: NUP107
• Gene ID (NCBI): 57122
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IF	Nat Commun; The master energy homeostasis regulator PGC-1α exhibits an mRNA nuclear export function; Authors - Simeon R Mihaylov
rat	WB	Neuron; Nucleoporin Seh1 Interacts with Olig2/Brd7 to Promote Oligodendrocyte Differentiation and Myelination.; Authors - Zhixiong Liu
mouse	WB	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu
mouse	WB	Elife; Suppression of C9orf72 RNA repeat-induced neurotoxicity by the ALS-associated RNA-binding protein Zfp106.; Authors - Barbara Celona
mouse	WB	Exp Gerontol; Age-dependent changes in nuclear-cytoplasmic signaling in skeletal muscle.; Authors - Shama R Iyer
human	IF	Cell Rep; Antiviral Immune Response as a Trigger of FUS Proteinopathy in Amyotrophic Lateral Sclerosis.; Authors - Tatyana A Shelkovnikova

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Barbara (Verified Customer) (03-28-2022)

Reliable antibody for western blot detection of NUP-107

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Neuro-2A lysates