NUP133 Rekombinanter Antikörper

NUP133 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

243092B5

Kat-Nr. : 85609-1-RR

Synonyme

133 kDa nucleoporin, hNUP133, nucleoporin 133kDa, Nucleoporin Nup133

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  WB analysis using 85609-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85609-1-RR (NUP133 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85609-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85609-1-RR against Human NUP133 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85609-1-RR bindet in WB, ELISA NUP133 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NUP133 fusion protein Ag3100
• Vollständiger Name: nucleoporin 133kDa
• Berechnetes Molekulargewicht: 1156 aa, 129 kDa
• Beobachtetes Molekulargewicht: 129 kDa
• GenBank-Zugangsnummer: BC020107
• Gene symbol: NUP133
• Gene ID (NCBI): 55746
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear pore complex protein Nup133 is a protein that in humans is encoded by the NUP133 gene. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells.