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NUP153 Polyklonaler Antikörper

NUP153 Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14189-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of K-562 cells using NUP153 Polyclonal antibody (14189-1-AP)

    WB analysis of K-562 using 14189-1-AP (same clone as 14189-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 14189-1-AP (NUP153 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14189-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NUP153 Polyclonal antibody (14189-1-AP)

    WB analysis using 14189-1-AP (same clone as 14189-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14189-1-AP (NUP153 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14189-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using NUP153 Polyclonal antibody (14189-1-AP)

    WB analysis of A431 using 14189-1-AP (same clone as 14189-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 14189-1-AP (NUP153 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14189-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using NUP153 Polyclonal antibody (14189-1-AP)

    IP experiment of K-562 using 14189-1-AP (same clone as 14189-1-PBS)

    IP result of anti-NUP153 (IP:14189-1-AP, 4ug; Detection:14189-1-AP 1:500) with K-562 cells lysate 3600ug. This data was developed using the same antibody clone with 14189-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

14189-1-PBS bindet in WB, IP, Indirect ELISA NUP153 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen NUP153 fusion protein Ag5519
Vollständiger Name nucleoporin 153kDa
Berechnetes Molekulargewicht 154 kDa
Beobachtetes Molekulargewicht 154 kDa
GenBank-ZugangsnummerBC052965
Gene symbol NUP153
Gene ID (NCBI) 9972
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NUP153 is an FG-repeat containing NUP, contains a high-affinity site for importin-β, localizes on the nucleoplasmic face of the nuclear pore and is required for assembly of the nuclear basket and import of a subset of nuclear proteins [PMID:18845677]. NUP153 also facilitates nuclear export of mRNA and ribonucleoprotein particles and may have additional functions within the nucleus [PMID:16195343]. The 53BP1-NUP153/importin-β pathway as an important aspect of the DDR network add to an emerging evidence of subcellular trafficking as an integral part of genome surveillance [PMID:22075984].