NUP153 Rekombinanter Antikörper

NUP153 Rekombinant Antikörper für WB, IF/ICC, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251519G11

Kat-Nr. : 86622-1-PBS

Synonyme

153 kDa nucleoporin, HNUP153, N153, nucleoporin 153kDa, Nucleoporin Nup153

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  WB analysis using 86622-1-RR (same clone as 86622-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86622-1-RR (NUP153 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86622-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02679-1

  The mean NUP153 concentration was determined to be 30.07 ng/mL in K-562 cell extract based on a 1.20 mg/mL extract load, 97.51 ng/mL in HepG2 cell extract based on a 1.20 mg/mL extract load and 33.07 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02679-1

  Sandwich ELISA standard curve of MP02679-1, Human NUP153 Recombinant Matched Antibody Pair - PBS only. 86622-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag5519. 86622-2-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL.

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  IF Staining of HeLa using 86622-1-RR (same clone as 86622-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUP153 antibody (86622-1-RR, Clone: 251519G11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86622-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86622-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86622-1-RR against Human NUP153 were performed. The affinity constant is 2.32 nM.

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Geprüfte Anwendungen

Produktinformation

86622-1-PBS bindet in WB, IF/ICC, Sandwich ELISA, Indirect ELISA NUP153 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NUP153 fusion protein Ag5519
• Vollständiger Name: nucleoporin 153kDa
• Berechnetes Molekulargewicht: 154 kDa
• Beobachtetes Molekulargewicht: 160-170 kDa
• GenBank-Zugangsnummer: BC052965
• Gene symbol: NUP153
• Gene ID (NCBI): 9972
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NUP153 is an FG-repeat containing NUP, contains a high-affinity site for importin-β, localizes on the nucleoplasmic face of the nuclear pore and is required for assembly of the nuclear basket and import of a subset of nuclear proteins [PMID:18845677]. NUP153 also facilitates nuclear export of mRNA and ribonucleoprotein particles and may have additional functions within the nucleus [PMID:16195343]. The 53BP1-NUP153/importin-β pathway as an important aspect of the DDR network add to an emerging evidence of subcellular trafficking as an integral part of genome surveillance [PMID:22075984].