NUP205 Polyklonaler Antikörper

NUP205 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 24439-1-AP

Synonyme

205 kDa nucleoporin, C7orf14, KIAA0225, nucleoporin 205kDa, Nucleoporin Nup205, NUP205

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 24439-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24439-1-AP (NUP205 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24439-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24439-1-AP (NUP205 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 24439-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24439-1-AP (NUP205 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 24439-1-AP

  IP Result of anti-NUP205 (IP:24439-1-AP, 4ug; Detection:24439-1-AP 1:1500) with HeLa cells lysate 4000ug.

• 

  IHC staining of human stomach using 24439-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach slide using 24439-1-AP (NUP205 Antibody) at dilution of 1:50.

• 

  IHC staining of human stomach using 24439-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach slide using 24439-1-AP (NUP205 Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

24439-1-AP bindet in WB, IP, IHC, IF, ELISA NUP205 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NUP205 fusion protein Ag19832
• Vollständiger Name: nucleoporin 205kDa
• Berechnetes Molekulargewicht: 2012 aa, 228 kDa
• Beobachtetes Molekulargewicht: 205 kDa
• GenBank-Zugangsnummer: BC136624
• Gene symbol: NUP205
• Gene ID (NCBI): 23165
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NUP205, also named as C7orf14 and KIAA0225, is a 205 kDa nucleoporin. It plays a role in the nuclear pore complex (NPC) assembly and/or maintenance.

Publikationen

Species	Application	Title
human	IF	Nat Methods; Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).; Authors - Marine H Laporte
mouse	WB	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu
human	WB	Oncogene; DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.; Authors - Bin Chen
human	WB	Hum Mutat; Lamin A mutation impairs interaction with nucleoporin NUP155 and disrupts nucleocytoplasmic transport in atrial fibrillation.; Authors - Meng Han
mouse	IF	Cell Rep Methods; TissUExM enables quantitative ultrastructural analysis in whole vertebrate embryos by expansion microscopy; Authors - Emmanuelle Steib
human	WB,IHC	Front Oncol; Exploring the relationship between abnormally high expression of NUP205 and the clinicopathological characteristics, immune microenvironment, and prognostic value of lower-grade glioma; Authors - Wenjia Liang