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NUP214 Polyklonaler Antikörper

NUP214 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24113-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using NUP214 Polyclonal antibody (24113-1-AP)

    WB analysis using 24113-1-AP (same clone as 24113-1-PBS)

    K-562 cells were subjected to Tris-Acetate gel system followed by western blot with 24113-1-AP (NUP214 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24113-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using NUP214 Polyclonal antibody (24113-1-AP)

    IHC staining of mouse kidney using 24113-1-AP (same clone as 24113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24113-1-AP (NUP214 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24113-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using NUP214 Polyclonal antibody (24113-1-AP)

    IHC staining of mouse kidney using 24113-1-AP (same clone as 24113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24113-1-AP (NUP214 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24113-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

24113-1-PBS bindet in WB, IHC, Indirect ELISA NUP214 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen NUP214 fusion protein Ag21377
Vollständiger Name nucleoporin 214kDa
Berechnetes Molekulargewicht 2080 aa, 213 kDa
Beobachtetes Molekulargewicht250 kDa
GenBank-ZugangsnummerBC105998
Gene symbol NUP214
Gene ID (NCBI) 8021
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-affinitätsgereinigt
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NUP214 is also known as CAN and is an FG nucleoporin anchored to the cytoplasmic ring of the nuclear pore complex (NPC) and forms a subcomplex with nucleoporin NUP88. NUP214 is a component of the NPC with a key role in protein and mRNA nuclear export. NUP214 is a target for chromosomal translocations involved in leukemogenesis (PMID: 17264208, 30669574). NUP214 is expressed in thymus, spleen, bone marrow, kidney, brain, and testis, but hardly in all other tissues or in whole embryos during development. The observed molecular weight of NUP214 is 250 kDa, which is consistent with the description in the literature (PMID: 12191473).