NUP50 Polyklonaler Antikörper

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• KD/KO Validated

NUP50 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 20798-1-AP

Synonyme

50 kDa nucleoporin, nucleoporin 50kDa, Nucleoporin Nup50, NUP50

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  WB analysis of Jurkat using 20798-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20798-1-AP (NUP50 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20798-1-AP

  WB result of NUP50 antibody (20798-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUP50 transfected HEK-293 cells.

• 

  WB analysis of mouse brain using 20798-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20798-1-AP (NUP50 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 20798-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20798-1-AP (NUP50 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20798-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20798-1-AP (NUP50 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 20798-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20798-1-AP (NUP50 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 20798-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20798-1-AP (NUP50 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:2500-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below

Produktinformation

20798-1-AP bindet in WB, IHC, ELISA NUP50 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NUP50 fusion protein Ag14710
• Vollständiger Name: nucleoporin 50kDa
• Berechnetes Molekulargewicht: 468 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa, 65 kDa
• GenBank-Zugangsnummer: BC028125
• Gene symbol: NUP50
• Gene ID (NCBI): 10762
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nup50 (also known as Npap60) is a nucleoporin that binds directly to importin α and their interaction plays an important role in nuclear import. Nup50 is a mobile nucleoporin localized primarily in the nuclear basket of the nuclear pore complex (NPC) and in the nucleoplasm. Human Nup50 has two isoforms: Npap60L (1-469 aa), and its alternatively spliced isoform, Npap60S (29-469 aa) of Npap60L. This antibody recognizes both of two isoforms.

Publikationen

Species	Application	Title
human	WB	Oncogene; DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.; Authors - Bin Chen
rat	WB	J Cell Sci; Nucleoporin 50 mediates Kcna4 transcription to regulate cardiac electrical activity.; Authors - Xueting Gao ; KD Validated
human	WB	Nat Phys; Structural anisotropy results in mechano-directional transport of proteins across nuclear pores; Authors - Fani Panagaki ; KD Validated