NUP54 Polyklonaler Antikörper

NUP54 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16232-1-PBS

Synonyme

Nucleoporin p54, nucleoporin 54kDa, 54 kDa nucleoporin

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  WB analysis of HeLa using 16232-1-AP (same clone as 16232-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16232-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 16232-1-AP (same clone as 16232-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16232-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 16232-1-AP (same clone as 16232-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16232-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 16232-1-AP (same clone as 16232-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16232-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 16232-1-AP (same clone as 16232-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16232-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 16232-1-AP (same clone as 16232-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16232-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 16232-1-AP (same clone as 16232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16232-1-AP (NUP54 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16232-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 16232-1-AP (same clone as 16232-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16232-1-AP (NUP54 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16232-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16232-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA NUP54 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NUP54 fusion protein Ag8882
• Vollständiger Name: nucleoporin 54kDa
• Berechnetes Molekulargewicht: 507 aa, 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC012559
• Gene symbol: NUP54
• Gene ID (NCBI): 53371
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.