NUP62 Polyklonaler Antikörper

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• KD/KO Validated

NUP62 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(19)

Kat-Nr. : 13916-1-AP

Synonyme

SNDI, Nucleoporin Nup62, nucleoporin 62kDa, Nuclear pore glycoprotein p62, IBSN

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  WB analysis of PC-3 using 13916-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13916-1-AP (NUP62 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 13916-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13916-1-AP (NUP62 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 13916-1-AP

  IP result of anti-NUP62 (IP:13916-1-AP, 4ug; Detection:13916-1-AP 1:1000) with NIH/3T3 cells lysate 1200ug.

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  IHC staining of human stomach using 13916-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13916-1-AP (NUP62 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Produktinformation

13916-1-AP bindet in WB, IHC, IF, IP, ELISA NUP62 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NUP62 fusion protein Ag4912
• Vollständiger Name: nucleoporin 62kDa
• Berechnetes Molekulargewicht: 522 aa, 53 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: BC014842
• Gene symbol: NUP62
• Gene ID (NCBI): 23636
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NUP62 belongs to the nucleoporin NSP1/NUP62 family. It is essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex. Defects in NUP62 are the cause of infantile striatonigral degeneration (SNDI). NUP62 consists of a single promoter with a CpG island and three transcribed exons. It is ubiquitously expressed, an observation compatible with its essential role in transporting cargo across the nuclear envelope. (PMID:16029492)

Publikationen

Species	Application	Title
human	WB,IF	Nat Microbiol; Nuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus.; Authors - Adarsh Dharan
mouse	WB	Mol Cell; RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages; Authors - Teodorus Theo Susanto
mouse	WB	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu
human	WB	PLoS Genet; Nucleoporin TPR promotes tRNA nuclear export and protein synthesis in lung cancer cells.; Authors - Miao Chen
mouse	WB	iScience; Tat-P combined with GAPR1 releases Beclin1 to promote autophagy and improve Bronchopulmonary dysplasia model; Authors - Yahui Zhou
rat	WB	Pain; Transcriptional repression of the M channel subunit Kv7.2 in chronic nerve injury.; Authors - Rose Kirstin K