NUP62 Monoklonaler Antikörper

NUP62 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2G12B3

Kat-Nr. : 66573-1-Ig

Synonyme

Nucleoporin Nup62, nucleoporin 62kDa, Nuclear pore glycoprotein p62, IBSN, 62 kDa nucleoporin

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  WB analysis using 66573-1-Ig

  Various cells were subjected to SDS PAGE followed by western blot with 66573-1-Ig (NUP62 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 66573-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66573-1-Ig (NUP62 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 66573-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66573-1-Ig (NUP62 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66573-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66573-1-Ig (NUP62 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, DU 145-Zellen, HepG2-Zellen, PC-3-Zellen, Raji-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66573-1-Ig bindet in WB, IHC, ELISA NUP62 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NUP62 fusion protein Ag4912
• Vollständiger Name: nucleoporin 62kDa
• Berechnetes Molekulargewicht: 522 aa, 53 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: BC014842
• Gene symbol: NUP62
• Gene ID (NCBI): 23636
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NUP62 belongs to the nucleoporin NSP1/NUP62 family. It is essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex. Defects in NUP62 are the cause of infantile striatonigral degeneration (SNDI). NUP62 consists of a single promoter with a CpG island and three transcribed exons. It is ubiquitously expressed, an observation compatible with its essential role in transporting cargo across the nuclear envelope. (PMID:16029492)