NUP85 Polyklonaler Antikörper

NUP85 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15027-1-PBS

Synonyme

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  WB analysis using 15027-1-AP (same clone as 15027-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15027-1-AP (NUP85 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15027-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 15027-1-AP (same clone as 15027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15027-1-AP (NUP85 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15027-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 15027-1-AP (same clone as 15027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15027-1-AP (NUP85 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15027-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 15027-1-AP (same clone as 15027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15027-1-AP (NUP85 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15027-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 15027-1-AP (same clone as 15027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15027-1-AP (NUP85 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15027-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 15027-1-AP (same clone as 15027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15027-1-AP (NUP85 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15027-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 15027-1-AP (same clone as 15027-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NUP85 antibody (15027-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 15027-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 15027-1-AP (same clone as 15027-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NUP85 antibody (15027-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 15027-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15027-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA NUP85 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NUP85 fusion protein Ag7158
• Vollständiger Name: nucleoporin 85kDa
• Berechnetes Molekulargewicht: 75 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC000697
• Gene symbol: NUP85
• Gene ID (NCBI): 79902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

This gene encodes a protein component of the Nup107-160 subunit of the nuclear pore complex. NPCs are key for nucleocytoplasmic transport, but also regulate the mitotic machinery, transcription and chromatin organization through transport-independent mechanisms. The NUP107-160 complex is acknowledged to contribute to the assembly and maintenance of the NPC structure. Members of this largest subcomplex of the NPC associate with kinetochores, mitotic spindles, centrosomes and mitotic checkpoint regulators for proper completion of the cell cycle . Downregulation of NUP107-160 subcomplex members resulted in defective cytokinesis, compromised microtubule structures, altered cytoskeletal dynamics, impaired chromosome segregation and differentiation. (PMID:34170319)