NUP85 Rekombinanter Antikörper

NUP85 Rekombinant Antikörper für IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240116A2

Kat-Nr. : 83288-1-PBS

Synonyme

240116A2, 85 kDa nucleoporin, FROUNT, nucleoporin 85kDa, Nucleoporin Nup75

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  Sandwich ELISA standard curve of MP00330-1

  Sandwich ELISA standard curve of MP00330-1, Human NUP85 Recombinant Matched Antibody Pair - PBS only. 83288-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7086. 83288-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 ng/mL.

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  Sample test of MP00330-1

  The mean NUP85 concentration was determined to be 1,874.5 ng/mL in mouse liver tissue extract based on a 3.0 mg/mL extract load.

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  IF Staining of HepG2 using 83288-1-RR (same clone as 83288-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NUP85 antibody (83288-1-RR, Clone: 240116A2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83288-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 83288-1-RR (same clone as 83288-1-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug NUP85 Recombinant antibody (83288-1-RR, Clone:240116A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83288-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00330-1

  Cytometric bead array standard curve of MP00330-1, NUP85 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83288-2-PBS. Detection antibody: 83288-1-PBS. Standard: Ag7086. Range: 0.313-40 ng/mL.

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Geprüfte Anwendungen

Produktinformation

83288-1-PBS bindet in IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test NUP85 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NUP85 fusion protein Ag7086
• Vollständiger Name: nucleoporin 85kDa
• Berechnetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC000697
• Gene symbol: NUP85
• Gene ID (NCBI): 79902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

This gene encodes a protein component of the Nup107-160 subunit of the nuclear pore complex. NPCs are key for nucleocytoplasmic transport, but also regulate the mitotic machinery, transcription and chromatin organization through transport-independent mechanisms. The NUP107-160 complex is acknowledged to contribute to the assembly and maintenance of the NPC structure. Members of this largest subcomplex of the NPC associate with kinetochores, mitotic spindles, centrosomes and mitotic checkpoint regulators for proper completion of the cell cycle . Downregulation of NUP107-160 subcomplex members resulted in defective cytokinesis, compromised microtubule structures, altered cytoskeletal dynamics, impaired chromosome segregation and differentiation. (PMID:34170319)