NUPR1 Polyklonaler Antikörper

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• KD/KO Validated

NUPR1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 15056-1-AP

Synonyme

Candidate of metastasis 1, COM1, nuclear protein 1, NUPR1, P8, Protein p8

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Galerie der Validierungsdaten

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  WB analysis of L02 using 15056-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15056-1-AP (NUPR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 15056-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15056-1-AP (NUPR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15056-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15056-1-AP (NUPR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15056-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15056-1-AP (NUPR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15056-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15056-1-AP (NUPR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15056-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15056-1-AP (NUPR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15056-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15056-1-AP (NUPR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:300-1:600
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 5 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

15056-1-AP bindet in WB, IHC, IF, CoIP, ELISA NUPR1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NUPR1 fusion protein Ag7039
• Vollständiger Name: nuclear protein 1
• Berechnetes Molekulargewicht: 8 kDa
• Beobachtetes Molekulargewicht: 8 kDa, 17 kDa, 38 kDa
• GenBank-Zugangsnummer: BC002434
• Gene symbol: NUPR1
• Gene ID (NCBI): 26471
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NUPR1, also named as COM1 and P8, is a small nuclear protein that is responsive to various stress stimuli. NUPR1 can be induced by hypoxia, oxidative stress, while the expression is low in physiological condition. It is associated with chemoresistance in multiple malignancies. The predicted MW of NUPR1 is 8 kDa and we also detectet bands around 17 and 38 kDa, which are similar to the papers(PMID: 29130426, 27336713, 28694771, 22961798). NUPR1 was also found in a complex with ANXA2, TRIM21, YBX1, S100A9, HSPA8, HSPA9, HSPA4, HSPA5, and ESR1, suggesting that these proteins might play a role in its functional regulation (PMID: 33542201), and it can also form a complex with PADI4 and plays crucial functions in modulating DNA-repair, favoring metastasis, or facilitating citrullination of other proteins (PMID: 36858171).

Publikationen

Species	Application	Title
human,mouse	WB,IHC,IF,CoIP	BMC Med; NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma; Authors - Yizhi Zhan ; KD Validated
human	WB	PLoS One; Adenomatous Polyposis Coli loss controls cell cycle regulators and response to paclitaxel in MDA-MB-157 metaplastic breast cancer cells.; Authors - Emily M Astarita
human,mouse	IHC,CoIP	Cell Death Discov; The interplay of transcriptional coregulator NUPR1 with SREBP1 promotes hepatocellular carcinoma progression via upregulation of lipogenesis; Authors - Yongjia Wang ; KO Validated
human	WB,IHC	Cancer Med; NUPR1 imparts oncogenic potential in bladder cancer; Authors - Lifeng Zhang ; KD Validated
human	WB	PPAR Res; Nuclear Protein 1 Expression Is Associated with PPARG in Bladder Transitional Cell Carcinoma; Authors - Chao Lu
human	WB	Biochem Genet; WTAP Mediates NUPR1 Regulation of LCN2 Through m6A Modification to Influence Ferroptosis, Thereby Promoting Breast Cancer Proliferation, Migration and Invasion; Authors - Miduo Tan