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NWD1 Polyklonaler Antikörper

NWD1 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25025-1-PBS

Synonyme

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  • Western Blot (WB) analysis of SH-SY5Y cells using NWD1 Polyclonal antibody (25025-1-AP)

    WB analysis of SH-SY5Y using 25025-1-AP (same clone as 25025-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25025-1-AP (NWD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25025-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using NWD1 Polyclonal antibody (25025-1-AP)

    WB analysis of HepG2 using 25025-1-AP (same clone as 25025-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25025-1-AP (NWD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25025-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using NWD1 Polyclonal antibody (25025-1-AP)

    WB analysis of mouse brain using 25025-1-AP (same clone as 25025-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25025-1-AP (NWD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25025-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NWD1 Polyclonal antibody (25025-1-AP)

    IF Staining of HepG2 using 25025-1-AP (same clone as 25025-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 25025-1-AP (NWD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25025-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

25025-1-PBS bindet in WB, IF/ICC, Indirect ELISA NWD1 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen NWD1 fusion protein Ag21754
Vollständiger Name NACHT and WD repeat domain containing 1
Berechnetes Molekulargewicht 1564 aa, 175 kDa
Beobachtetes Molekulargewicht 77 kDa
GenBank-ZugangsnummerBC117698
Gene symbol NWD1
Gene ID (NCBI) 284434
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NWD1 also named as NWD1 Ens or NACHT and WD repeat domain containing 1 is a 1564 amino acid protein, which contains 1 NACHT domain and 13 WD repeat. The molecular weight of NWD1 was detected by western blot. The result shows that the molecular weight of NWD1 is 77 kDa., but NWD1 is a 174 kDa protein according to UniProt,. The function of NWD1 is still non-known, meanwhile the molecular weight of NWD1 is still remained to be confirmed.