NXF1 Monoklonaler Antikörper

NXF1 Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

2C2H1

Kat-Nr. : 67914-1-Ig

Synonyme

DKFZp667O0311, MEX67, mRNA export factor TAP, nuclear RNA export factor 1, NXF1, TAP, Tip associated protein, Tip associating protein

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Galerie der Validierungsdaten

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  WB analysis using 67914-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67914-1-Ig (NXF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 67914-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NXF1 antibody (67914-1-Ig, Clone: 2C2H1 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF): IF : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67914-1-Ig bindet in WB, IF, ELISA NXF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NXF1 fusion protein Ag27329
• Vollständiger Name: nuclear RNA export factor 1
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC004904
• Gene symbol: NXF1
• Gene ID (NCBI): 10482
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear RNA export factor 1 (NXF1, synonyms: TAP, MEX67) is one member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1(NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. This protein shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. NXF1 overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses.