NXN Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NXN Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 16128-1-AP

Synonyme

NRX, nucleoredoxin, NXN, TRG 4

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 16128-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16128-1-AP (NXN antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16128-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16128-1-AP (NXN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 16128-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16128-1-AP (NXN antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16128-1-AP

  IP Result of anti-NXN (IP:16128-1-AP, 4ug; Detection:16128-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human colon cancer using 16128-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16128-1-AP (NXN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16128-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16128-1-AP (NXN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 16128-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NXN antibody (16128-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, HCT 116-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Herzgewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

16128-1-AP bindet in WB, IP, IHC, IF, ELISA NXN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NXN fusion protein Ag9113
• Vollständiger Name: nucleoredoxin
• Berechnetes Molekulargewicht: 435 aa, 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC009327
• Gene symbol: NXN
• Gene ID (NCBI): 64359
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nucleoredoxin (NRX/NXN), a relatively uncharacterized member of the TRX family protein, has recently been reported to regulate the Wnt/β-catenin pathway, which itself regulates cell fate and early development, in a redox-dependent manner(PMID:17567240). Northern blot analysis detected several nucleoredoxin splice variants in adult mouse tissues, with highest expression in testis and skin and lowest expression in liver(PMID:9119370). It has 3 isoforms produced by alternative splicing. This antibody is specific to NXN.

Publikationen

Species	Application	Title
mouse	WB	Sci Signal; Mitochondrial reactive oxygen species promote epidermal differentiation and hair follicle development.; Authors - Hamanaka Robert B RB
human	WB	Free Radic Biol Med; Oxidative stress as a candidate mechanism for accelerated neuroectodermal differentiation due to trisomy 21.; Authors - Kendra M Prutton
human,mouse,rat	WB	FASEB J; A crucial role for Nox 1 in redox-dependent regulation of Wnt-β-catenin signaling.; Authors - Kajla Seheli S
human	WB	Antioxidants (Basel); Enzymatic Depletion of Mitochondrial Inorganic Polyphosphate (polyP) Increases the Generation of Reactive Oxygen Species (ROS) and the Activity of the Pentose Phosphate Pathway (PPP) in Mammalian Cells.; Authors - Vedangi Hambardikar
human	WB,IF	Antioxidants (Basel); Nucleoredoxin Plays a Key Role in the Maintenance of Retinal Pigmented Epithelium Differentiation.; Authors - Mariana I Holubiec ; KD Validated
human	IHC	Cell Death Dis; NXN suppresses metastasis of hepatocellular carcinoma by promoting degradation of Snail through binding to DUB3.; Authors - Yuanping Zhang