NXN Polyklonaler Antikörper

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• KD/KO Validated

NXN Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16128-1-PBS

Synonyme

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  WB analysis of HeLa using 16128-1-AP (same clone as 16128-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16128-1-AP (NXN antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16128-1-AP (same clone as 16128-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16128-1-AP (NXN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 16128-1-AP (same clone as 16128-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 16128-1-AP (NXN antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 16128-1-AP (same clone as 16128-1-PBS)

  IP result of anti-NXN (IP:16128-1-AP, 4ug; Detection:16128-1-AP 1:500) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 16128-1-AP (same clone as 16128-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16128-1-AP (NXN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 16128-1-AP (same clone as 16128-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16128-1-AP (NXN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 16128-1-AP (same clone as 16128-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NXN antibody (16128-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 16128-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16128-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA NXN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NXN fusion protein Ag9113
• Vollständiger Name: nucleoredoxin
• Berechnetes Molekulargewicht: 435 aa, 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC009327
• Gene symbol: NXN
• Gene ID (NCBI): 64359
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nucleoredoxin (NRX/NXN), a relatively uncharacterized member of the TRX family protein, has recently been reported to regulate the Wnt/β-catenin pathway, which itself regulates cell fate and early development, in a redox-dependent manner(PMID:17567240). Northern blot analysis detected several nucleoredoxin splice variants in adult mouse tissues, with highest expression in testis and skin and lowest expression in liver(PMID:9119370). It has 3 isoforms produced by alternative splicing. This antibody is specific to NXN.