NXT1 Monoklonaler Antikörper

NXT1 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

2C10A2

Kat-Nr. : 67680-1-Ig

Synonyme

MTR2, NTF2 like export factor 1, NTF2 related export protein 1, NXT1, P15, Protein p15

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  WB analysis using 67680-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67680-1-Ig (NXT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 67680-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67680-1-Ig (NXT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 67680-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NXT1 antibody (67680-1-Ig, Clone: 2C10A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

67680-1-Ig bindet in WB, IF/ICC, ELISA NXT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NXT1 fusion protein Ag30039
• Vollständiger Name: NTF2-like export factor 1
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC000759
• Gene symbol: NXT1
• Gene ID (NCBI): 29107
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NXT1 is located in the nuclear envelope and is homologous to nuclear transport factor 2. NXT1 functions as a nuclear export factor in both RAN (Ras-related nuclear protein)- and CRM1 (required for chromosome region maintenance)-dependent pathways. It is found to stimulate the export of U1 snRNA in RAN- and CRM1-dependent pathways and the export of tRNA and mRNA in a CRM1-independent pathway. NXT1 also heterodimerizes with Tap protein and regulates the ability of Tap protein to mediate nuclear mRNA export.

Publikationen

Species	Application	Title
human	WB	iScience; Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.; Authors - Mio Iwasaki