NY-ESO-1 Polyklonaler Antikörper

NY-ESO-1 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19521-1-PBS

Synonyme

CTAG1A, Autoimmunogenic cancer/testis antigen NY-ESO-1, CTAG, CTAG1, ESO1

---

• 

  WB analysis of human testis using 19521-1-AP (same clone as 19521-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 19521-1-AP (CTAG1A antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19521-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-1080 using 19521-1-AP (same clone as 19521-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 19521-1-AP (NY-ESO-1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19521-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 19521-1-AP (same clone as 19521-1-PBS)

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 19521-1-AP (NY-ESO-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19521-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 19521-1-AP (same clone as 19521-1-PBS)

  IP result of anti-NY-ESO-1 (IP:19521-1-AP, 4ug; Detection:19521-1-AP 1:500) with HepG2 cells lysate 3200ug. This data was developed using the same antibody clone with 19521-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 19521-1-AP (same clone as 19521-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19521-1-AP (NY-ESO-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19521-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 19521-1-AP (same clone as 19521-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19521-1-AP (NY-ESO-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19521-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

19521-1-PBS bindet in WB, IHC, IP, Indirect ELISA NY-ESO-1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cancer/testis antigen 1A
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 16-25 kDa
• GenBank-Zugangsnummer: NM_139250
• Gene symbol: NY-ESO-1
• Gene ID (NCBI): 246100
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CTAG1A, also named as NY-ESO-1, CTAG, CTAG1, ESO1, LAGE2, LAGE2A, CTAG1B, LAGE2B and CT6.1, belongs to the CTAG family.