Napsin A Monoklonaler Antikörper

Napsin A Monoklonal Antikörper für IF/ICC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H7F2

Kat-Nr. : CL488-60259

Synonyme

Asp 4, ASP4, Aspartyl protease 4, KAP, Kdap, NAP1, NAPA, NAPSA, Napsin 1, Napsin A, napsin A aspartic peptidase, Pronapsin A, SNAPA, TA01/TA02

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  IF Staining of HUVEC using CL488-60259

  Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using CL488-60259 (Napsin A antibody) at dilution of 1:50.

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  IF Staining of HUVEC using CL488-60259

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using CoraLite® Plus 488 Napsin A antibody (CL488-60259, Clone: 1H7F2 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HUVEC-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-60259 bindet in IF/ICC Napsin A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Napsin A fusion protein Ag9721
• Vollständiger Name: napsin A aspartic peptidase
• Berechnetes Molekulargewicht: 420 aa, 45 kDa
• GenBank-Zugangsnummer: BC017842
• Gene symbol: Napsin A
• Gene ID (NCBI): 9476
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Napsin is found in two isoforms, napsin A and B, with highly homologous nucleotide sequences (91.2%). Napsin A appears to be a functional proteinase, predominantly expressed in lung and kidney. Napsin B is transcribed exclusively in cells related to the immune system and lacks an in-frame stop codon and is believed to be a pseudogene.(PMID:12698189). Napsin A is superior to TTF-1 in distinguishing primary lung ACA from other carcinomas (except kidney), particularly primary lung small cell carcinoma, and primary thyroid carcinoma.(PMID:22288963).