NCAM1/CD56 Rekombinanter Antikörper

NCAM1/CD56 Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240936B9

Kat-Nr. : 83883-2-PBS

Synonyme

CD56, Ncam1, 240936B9, E NCAM, Ncam

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  WB analysis of C6 using 83883-2-RR (same clone as 83883-2-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 83883-2-RR (Ncam1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83883-2-PBS in a different storage buffer formulation.

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  WB analysis using 83883-2-RR (same clone as 83883-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83883-2-RR (NCAM1/CD56 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83883-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00841-1

  Cytometric bead array standard curve of MP00841-1, MOUSE NCAM1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83883-2-PBS. Detection antibody: 83883-4-PBS. Standard: Eg0652. Range: 0.469-60 ng/mL.

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  Affinity and Kinetic Characterization of 83883-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83883-2-RR against Mouse Ncam1 were performed. The affinity constant is 0.833 nM.

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Geprüfte Anwendungen

Produktinformation

83883-2-PBS bindet in WB, Cytometric bead array, Indirect ELISA NCAM1/CD56 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NCAM1/CD56 fusion protein Eg0652
• Vollständiger Name: neural cell adhesion molecule 1
• Berechnetes Molekulargewicht: 119 kDa
• Beobachtetes Molekulargewicht: 120 kDa, 140 kDa, 180 kDa
• Gene symbol: Ncam1
• Gene ID (NCBI): 17967
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells, and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by the addition of polysialic acid (PSA) (PMID: 14976519). Several other isoforms have also been described (PMID: 1856291).