NCAM1/CD56 Rekombinanter Antikörper

NCAM1/CD56 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240936B9

Kat-Nr. : 83883-2-RR

Synonyme

Ncam1, CD56, 240936B9, NCAM 1, Ncam

---

• 

  WB analysis of C6 using 83883-2-RR

  C6 cells were subjected to SDS PAGE followed by western blot with 83883-2-RR (Ncam1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83883-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83883-2-RR (NCAM1/CD56 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 83883-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83883-2-RR against Mouse Ncam1 were performed. The affinity constant is 0.833 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Cerebellum-Gewebe, C6-Zellen, Maushirngewebe, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83883-2-RR bindet in WB, ELISA NCAM1/CD56 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: neural cell adhesion molecule 1
• Berechnetes Molekulargewicht: 119 kDa
• Beobachtetes Molekulargewicht: 120 kDa, 140 kDa, 180 kDa
• Gene symbol: Ncam1
• Gene ID (NCBI): 17967
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells, and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by the addition of polysialic acid (PSA) (PMID: 14976519). Several other isoforms have also been described (PMID: 1856291).