NCAM1/CD56 Rekombinanter Antikörper

NCAM1/CD56 Rekombinant Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240936B10

Kat-Nr. : 83883-5-RR

Synonyme

CD56, Ncam1, 240936B10, E NCAM, Ncam

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  IHC staining of mouse brain using 83883-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83883-5-RR (NCAM1/CD56 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 83883-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83883-5-RR (NCAM1/CD56 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 83883-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83883-5-RR (NCAM1/CD56 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 83883-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83883-5-RR (NCAM1/CD56 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 83883-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83883-5-RR (NCAM1/CD56 antibody) at dilution of 1:9000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Neuro-2a using 83883-5-RR

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using Ncam1 antibody (83883-5-RR, Clone: 240936B10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of Neuro-2a using 83883-5-RR

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using Ncam1 antibody (83883-5-RR, Clone: 240936B10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83883-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83883-5-RR against Mouse Ncam1 were performed. The affinity constant is 0.184 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maushirngewebe, Maus-Cerebellum-Gewebe, Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Neuro-2a-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83883-5-RR bindet in IHC, IF/ICC, ELISA NCAM1/CD56 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NCAM1/CD56 fusion protein Eg0652
• Vollständiger Name: neural cell adhesion molecule 1
• Berechnetes Molekulargewicht: 119 kDa
• Beobachtetes Molekulargewicht: 120 kDa, 140 kDa, 180 kDa
• Gene symbol: Ncam1
• Gene ID (NCBI): 17967
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells, and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by the addition of polysialic acid (PSA) (PMID: 14976519). Several other isoforms have also been described (PMID: 1856291).