Nectin-2/CD112 Polyklonaler Antikörper

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• KD/KO Validated

Nectin-2/CD112 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 27171-1-AP

Synonyme

Nectin-2, Nectin cell adhesion molecule 2, Nectin 2, HVEB, Herpesvirus entry mediator B

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  WB analysis of HAP1 using 27171-1-AP

  WB result of Nectin-2/CD112 antibody (27171-1-AP; 1:4000; room temperature for 1.5 hours) with wild-type and Nectin-2/CD112 knockout HAP1 cells.

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  WB analysis using 27171-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27171-1-AP (Nectin-2/PVRL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 27171-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27171-1-AP (Nectin-2/PVRL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 27171-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 27171-1-AP (Nectin-2/PVRL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 27171-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27171-1-AP (Nectin-2/PVRL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 27171-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27171-1-AP (Nectin-2/PVRL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 27171-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27171-1-AP (Nectin-2/PVRL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 27171-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27171-1-AP (Nectin-2/PVRL2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 27171-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27171-1-AP (Nectin-2/PVRL2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27171-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27171-1-AP (Nectin-2/PVRL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27171-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27171-1-AP (Nectin-2/PVRL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattennierengewebe, Jurkat-Zellen, Mausnierengewebe, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Mammakarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 4 publications below

Produktinformation

27171-1-AP bindet in WB, IHC, IF, ELISA Nectin-2/CD112 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Nectin-2/CD112 fusion protein Ag26040
• Vollständiger Name: poliovirus receptor-related 2 (herpesvirus entry mediator B)
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC003091
• Gene symbol: Nectin-2/CD112
• Gene ID (NCBI): 5819
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nectin 2, also named as PVRL2, CD112, HVEB, PRR2 and PVRR2, is an adhesion molecule widely expressed in cell lines of different lineages, including hematopoietic, neuronal, endothelial and epithelial cells. CD112 belongs to a new family of immunoglobulin-like molecules that includes four members (CD111, CD112, PRR3 and CD155) sharing an ectodomain made of three Ig domains, of V and C types. CD112 is expressed in the myelo-monocytic and megakaryocytic hematopoietic lineages and the function in hematopoiesis is currently unknown. CD112 is an intercellular homophilic adhesion. CD112 localizes specifically at adherents junctions via its cytoplasmic interaction with the scaffold F-actin binding protein afadin. Disruption of the murine CD112 gene leads to infertility of male mice with morphologically aberrant spermatozoa. CD112 mediates entry of some alphaherpesvirus mutants (also named HveB) via its V domain. CD112 is involved in cell to cell spreading of viruses.

Publikationen

Species	Application	Title
mouse	IHC	Mucosal Immunol; Alpha-hemolysin of uropathogenic Escherichia coli induces GM-CSF-mediated acute kidney injury.; Authors - Changying Wang
rat	IF	J Transl Int Med; Lipid-related protein NECTIN2 is an important marker in the progression of carotid atherosclerosis: An intersection of clinical and basic studies.; Authors - Shen Li
human	IHC	iScience; Proteomic and single-cell landscape reveals novel pathogenic mechanisms of HBV-infected intrahepatic cholangiocarcinoma; Authors - Yifei Shen
human	IF	Sci Rep; Single-cell RNA sequencing unveils tumor heterogeneity and immune microenvironment between subungual and plantar melanoma; Authors - Panpan Wang
human	IF	Cancer Lett; Histone Lactylation Dynamics: Unlocking the Triad of Metabolism, Epigenetics, and Immune Regulation in Metastatic Cascade of Pancreatic Cancer; Authors - Xing Wang
mouse	WB	Neurosci Bull; Dorsal CA1 NECTIN3 Reduction Mediates Early-Life Stress-Induced Object Recognition Memory Deficits in Adolescent Female Mice; Authors - Yu-Nu Ma