Nectin-2/CD112 Rekombinanter Antikörper

Nectin-2/CD112 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241798A11

Kat-Nr. : 84439-6-RR

Synonyme

241798A11, CD112, Herpes virus entry mediator B, HveB, mHveB

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  WB analysis using 84439-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84439-6-RR (Nectin-2/CD112 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of RAW 264.7 using 84439-6-RR

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using Nectin-2/CD112 antibody (84439-6-RR, Clone: 241798A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, C2C12-Zellen, Maushodengewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF/ICC: RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84439-6-RR bindet in WB, IF/ICC, ELISA Nectin-2/CD112 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Nectin-2/CD112 fusion protein Eg2019
• Vollständiger Name: poliovirus receptor-related 2
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 65-75 kDa
• GenBank-Zugangsnummer: NM_008990.3
• Gene symbol: Nectin-2/CD112
• Gene ID (NCBI): 19294
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nectin 2, also named as PVRL2, CD112, HVEB, PRR2 and PVRR2, is an adhesion molecule widely expressed in cell lines of different lineages, including hematopoietic, neuronal, endothelial and epithelial cells. CD112 belongs to a new family of immunoglobulin-like molecules that includes four members (CD111, CD112, PRR3 and CD155) sharing an ectodomain made of three Ig domains, of V and C types. CD112 is expressed in the myelo-monocytic and megakaryocytic hematopoietic lineages and the function in hematopoiesis is currently unknown. CD112 is an intercellular homophilic adhesion. CD112 localizes specifically at adherents junctions via its cytoplasmic interaction with the scaffold F-actin binding protein afadin. Disruption of the murine CD112 gene leads to infertility of male mice with morphologically aberrant spermatozoa. CD112 mediates entry of some alphaherpesvirus mutants (also named HveB) via its V domain. CD112 is involved in cell to cell spreading of viruses.