Nectin-2/CD112 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

Nectin-2/CD112 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241725D3

Kat-Nr. : 84460-5-RR

Synonyme

Nectin-2, 241725D3, CD112, Herpes virus entry mediator B, Herpesvirus entry mediator B

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  WB analysis of HAP1 using 84460-5-RR

  WB result of Nectin-2/CD112 antibody (84460-5-RR; 1:2000; room temperature for 1.5 hours) with wild-type and Nectin-2/CD112 knockout HAP1 cells.

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  WB analysis using 84460-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84460-5-RR (Nectin-2/CD112 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of K-562 using 84460-5-RR

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using Nectin-2/CD112 antibody (84460-5-RR, Clone: 241725D3 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84460-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84460-5-RR against Human Nectin-2/CD112 were performed. The affinity constant is 0.314 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, A549-Zellen, MDA-MB-231-Zellen
• Erfolgreiche Detektion in IF/ICC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84460-5-RR bindet in WB, IF/ICC, ELISA Nectin-2/CD112 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Nectin-2/CD112 fusion protein Eg1670
• Vollständiger Name: poliovirus receptor-related 2 (herpesvirus entry mediator B)
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 65-75 kDa
• GenBank-Zugangsnummer: NM_001042724.2
• Gene symbol: Nectin-2/CD112
• Gene ID (NCBI): 5819
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nectin 2, also named as PVRL2, CD112, HVEB, PRR2 and PVRR2, is an adhesion molecule widely expressed in cell lines of different lineages, including hematopoietic, neuronal, endothelial and epithelial cells. CD112 belongs to a new family of immunoglobulin-like molecules that includes four members (CD111, CD112, PRR3 and CD155) sharing an ectodomain made of three Ig domains, of V and C types. CD112 is expressed in the myelo-monocytic and megakaryocytic hematopoietic lineages and the function in hematopoiesis is currently unknown. CD112 is an intercellular homophilic adhesion. CD112 localizes specifically at adherents junctions via its cytoplasmic interaction with the scaffold F-actin binding protein afadin. Disruption of the murine CD112 gene leads to infertility of male mice with morphologically aberrant spermatozoa. CD112 mediates entry of some alphaherpesvirus mutants (also named HveB) via its V domain. CD112 is involved in cell to cell spreading of viruses.