Nephrin Polyklonaler Antikörper

Nephrin Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 22912-1-AP

Synonyme

NPHS1, CNF, NPHN, NPHS 1, Renal glomerulus-specific cell adhesion receptor

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  WB analysis using 22912-1-AP

  Mouse kidney tissues were subjected to SDS PAGE followed by western blot with 22912-1-AP (Nephrin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 22912-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22912-1-AP (Nephrin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 22912-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22912-1-AP (Nephrin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 22912-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22912-1-AP (Nephrin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 22912-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22912-1-AP (Nephrin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 22912-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 22912-1-AP (Nephrin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat kidney using 22912-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat kidney tissue using Nephrin antibody (22912-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Rattennierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

22912-1-AP bindet in WB, IHC, IF-P, ELISA Nephrin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Nephrin fusion protein Ag18994
• Vollständiger Name: nephrosis 1, congenital, Finnish type (nephrin)
• Berechnetes Molekulargewicht: 1241 aa, 135 kDa
• Beobachtetes Molekulargewicht: 120-130 kDa
• GenBank-Zugangsnummer: BC156935
• Gene symbol: Nephrin
• Gene ID (NCBI): 4868
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Podocytes are a major component of the glomerular blood filtration barrier. Podocyte protein Nephrin, also known as NPHS1, is the first to be identified as a central component of the slit diaphragm (SD), which is the podocyte cell-cell junction. Mutations in Nephrin lead to congenital nephrosis, such as steroid-resistant proteinuria and congenital nephrotic syndrome of the Finnish type (CNF).

Publikationen

Species	Application	Title
mouse	WB	BMC Med Genomics; Deciphering the molecular nexus of BTG2 in periodontitis and diabetic kidney disease; Authors - Binhui Pan
human	IHC	J Nephrol; Association of the podocyte phenotype with extracapillary hypercellularity in patients with diabetic kidney disease; Authors - Weixia Han
rat	IF	J Ethnopharmacol; Supplemented Gegen Qinlian Decoction Formula attenuates podocyte mitochondrial fission and renal fibrosis in diabetic kidney disease by inhibiting TNF-α-mediated necroptosis, compared with empagliflozin; Authors - Yu Wang
mouse	WB	Antioxidants (Basel); Identification and Validation of the miR/RAS/RUNX2 Autophagy Regulatory Network in AngII-Induced Hypertensive Nephropathy in MPC5 Cells Treated with Hydrogen Sulfide Donors; Authors - Qing Ye
mouse	IF	Exp Cell Res; Fbxo11 maintains mitochondrial function and prevents podocyte injury in adriamycin-induced nephropathy by mediating the ubiquitin degradation of Fosl2; Authors - Yanhua Jin
rat	WB,IHC	Mitochondrion; The compound XueShuanTong promotes podocyte mitochondrial autophagy via the AMPK/mTOR pathway to alleviate diabetic nephropathy injury; Authors - Chuangbiao Zhang