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Nephrin Rekombinanter Antikörper

Nephrin Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250064G12

Kat-Nr. : 85845-1-PBS

Synonyme

NPHS1, CNF, NPHN, NPHS 1, Renal glomerulus-specific cell adhesion receptor

Filter:
  • Western Blot (WB) analysis of mouse kidney tissue using Nephrin Recombinant antibody (85845-1-RR)

    WB analysis of mouse kidney using 85845-1-RR (same clone as 85845-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 85845-1-RR (Nephrin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85845-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85845-1-RR

    Affinity and Kinetic Characterization of 85845-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85845-1-RR against Human Nephrin were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

85845-1-PBS bindet in WB, Indirect ELISA Nephrin und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Nephrin fusion protein Ag18994
Vollständiger Name nephrosis 1, congenital, Finnish type (nephrin)
Berechnetes Molekulargewicht 1241 aa, 135 kDa
Beobachtetes Molekulargewicht120-130 kDa
GenBank-ZugangsnummerBC156935
Gene symbol Nephrin
Gene ID (NCBI) 4868
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Podocytes are a major component of the glomerular blood filtration barrier. Podocyte protein Nephrin, also known as NPHS1, is the first to be identified as a central component of the slit diaphragm (SD), which is the podocyte cell-cell junction. Mutations in Nephrin lead to congenital nephrosis, such as steroid-resistant proteinuria and congenital nephrotic syndrome of the Finnish type (CNF).