Nephrin Rekombinanter Antikörper

Nephrin Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250064G12

Kat-Nr. : 85845-1-RR

Synonyme

NPHS1, CNF, NPHN, NPHS 1, Renal glomerulus-specific cell adhesion receptor

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  WB analysis of mouse kidney using 85845-1-RR

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 85845-1-RR (Nephrin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85845-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85845-1-RR against Human Nephrin were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85845-1-RR bindet in WB, ELISA Nephrin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Nephrin fusion protein Ag18994
• Vollständiger Name: nephrosis 1, congenital, Finnish type (nephrin)
• Berechnetes Molekulargewicht: 1241 aa, 135 kDa
• Beobachtetes Molekulargewicht: 120-130 kDa
• GenBank-Zugangsnummer: BC156935
• Gene symbol: Nephrin
• Gene ID (NCBI): 4868
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Podocytes are a major component of the glomerular blood filtration barrier. Podocyte protein Nephrin, also known as NPHS1, is the first to be identified as a central component of the slit diaphragm (SD), which is the podocyte cell-cell junction. Mutations in Nephrin lead to congenital nephrosis, such as steroid-resistant proteinuria and congenital nephrotic syndrome of the Finnish type (CNF).