Nesprin 3 Polyklonaler Antikörper

Nesprin 3 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (1)

Kat-Nr. : 27132-1-AP

Synonyme

C14orf49, Nesprin 3

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  WB analysis of C2C12 using 27132-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 27132-1-AP (Nesprin 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27132-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27132-1-AP (Nesprin 3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 27132-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 27132-1-AP (Nesprin 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 27132-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 27132-1-AP (Nesprin 3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C2C12-Zellen, Mausmilzgewebe, Rattenmilzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

27132-1-AP bindet in WB, IF, ELISA Nesprin 3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Nesprin 3 fusion protein Ag26002
• Vollständiger Name: chromosome 14 open reading frame 49
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC146604
• Gene symbol: Nesprin 3
• Gene ID (NCBI): 161176
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nesprin 3 also known as C14orf49, SYNE3, belongs to the Nesprin family. Nesprin-3 is a type II transmembrane protein of the outer nuclear membrane and contains a C-terminal KASH domain and a series of spectrin repeats(PMID: 16330710). Nesprin-3, a protein that links intermediate filaments to the nucleus, regulates endothelial cell morphology, perinuclear cytoskeletal organization, and flow-induced cell polarization and migration. Nesprin-3 is robustly expressed in human aortic endothelial cells and localizes to the nuclear envelope(PMID: 21937718).

Publikationen

Species	Application	Title
mouse	IF	Front Cell Dev Biol; SUN5 Interacting With Nesprin3 Plays an Essential Role in Sperm Head-to-Tail Linkage: Research on Sun5 Gene Knockout Mice.; Authors - Yunfei Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ning (Verified Customer) (01-28-2023)

Good antibody to prob nesprin 3.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse cortex lysate

Nesprin 3 Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:27132-1-AP)