Nesprin 3 Monoklonaler Antikörper

Nesprin 3 Monoklonal Antikörper für WB, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D1G12

Kat-Nr. : 68420-1-PBS

Synonyme

1D1G12, C14orf139, C14orf49, KASH domain-containing protein 3, KASH3

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  WB analysis using 68420-1-Ig (same clone as 68420-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68420-1-Ig (C14orf49 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68420-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 68420-1-Ig (same clone as 68420-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Nesprin 3 antibody (68420-1-Ig, Clone: 1D1G12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68420-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68420-1-Ig (same clone as 68420-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Nesprin 3 antibody (68420-1-Ig, Clone: 1D1G12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68420-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68420-1-PBS bindet in WB, IF/ICC, IF-P, Indirect ELISA Nesprin 3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Nesprin 3 fusion protein Ag26012
• Vollständiger Name: chromosome 14 open reading frame 49
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC146604
• Gene symbol: Nesprin 3
• Gene ID (NCBI): 161176
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nesprin 3 also known as C14orf49, SYNE3, belongs to the Nesprin family. Nesprin-3 is a type II transmembrane protein of the outer nuclear membrane and contains a C-terminal KASH domain and a series of spectrin repeats(PMID: 16330710). Nesprin-3, a protein that links intermediate filaments to the nucleus, regulates endothelial cell morphology, perinuclear cytoskeletal organization, and flow-induced cell polarization and migration. Nesprin-3 is robustly expressed in human aortic endothelial cells and localizes to the nuclear envelope(PMID: 21937718).