Nestin Monoklonaler Antikörper
Nestin Monoklonal Antikörper für WB, ELISA
Wirt / Isotyp
Maus / IgG2b
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, ELISA
Konjugation
Unkonjugiert
CloneNo.
1D11H2
Kat-Nr. : 66259-1-Ig
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Neuro-2a-Zellen, HEK-293-Zellen, PC-12-Zellen, Ratten-Cerebellum-Gewebe |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 15 publications below |
| FC | See 2 publications below |
Produktinformation
66259-1-Ig bindet in WB, ELISA Nestin und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Maus, Ratte |
| Wirt / Isotyp | Maus / IgG2b |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | Peptid |
| Vollständiger Name | nestin |
| Berechnetes Molekulargewicht | 177 kDa |
| Beobachtetes Molekulargewicht | 150-170 kDa |
| GenBank-Zugangsnummer | NM_006617 |
| Gene symbol | Nestin |
| Gene ID (NCBI) | 10763 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for Nestin antibody 66259-1-Ig | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Cell Metab Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells. | ||
Cell Res RNA m6A modification orchestrates a LINE-1-host interaction that facilitates retrotransposition and contributes to long gene vulnerability. | ||
J Allergy Clin Immunol Ras homolog family member A/Rho-associated protein kinase 1 signaling modulates lineage commitment of mesenchymal stem cells in asthmatic patients through lymphoid enhancer-binding factor 1. | ||
Small Stemness Maintenance and Massproduction of Neural Stem Cells on Poly L-Lactic Acid Nanofibrous Membrane Based on Piezoelectriceffect. | ||
EBioMedicine Silencing of circular RNA HIPK2 in neural stem cells enhances functional recovery following ischaemic stroke. | ||
Int J Biol Macromol A multifunctional polydopamine/genipin/alendronate nanoparticle licences fibrin hydrogels osteoinductive and immunomodulatory potencies for repairing bone defects |



