NeuN Polyklonaler Antikörper

NeuN Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Kat-Nr. : 23060-1-AP

Synonyme

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• 

  WB analysis of mouse brain using 23060-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23060-1-AP (NeuN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 23060-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23060-1-AP (NeuN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 3T3-L1 using 23060-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 23060-1-AP (NeuN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 23060-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23060-1-AP (NeuN Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse brain using 23060-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23060-1-AP (NeuN Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, 3T3-L1-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 4 publications below
• IF: See 8 publications below

Produktinformation

23060-1-AP bindet in WB, IHC, IF, ELISA NeuN und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, hamster, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NeuN fusion protein Ag19347
• Vollständiger Name: hexaribonucleotide binding protein 3
• Beobachtetes Molekulargewicht: 38-50 kDa
• GenBank-Zugangsnummer: BC093713
• Gene symbol: NeuN
• Gene ID (NCBI): 146713
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NeuN, also named as FOX3 and RBFOX3, is neuronal-specific nuclear protein with MW 38-50kd(~48kd). It is one of a family of 3 mammalian Fox homologues. FOX3(Or NeuN) functions in RNA-binding protein that regulates alternative splicing events.

Publikationen

Species	Application	Title
goat	IF	Cell Cycle; Effects of the histone acetylase inhibitor C646 on growth and differentiation of adipose-derived stem cells.; Authors - Zhimin Wang
hamster	IHC	Neurotherapeutics; PMID: 28083805; Authors - Syed Zahid Ali Shah
rat	IF	Exp Cell Res; CEBPD aggravates apoptosis and oxidative stress of neuron after ischemic stroke by Nrf2/HO-1 pathway; Authors - Nan Chen
rat	WB,IF	Neurochem Int; Tert-butylhydroquinone post-treatment attenuates neonatal hypoxic-ischemic brain damage in rats.; Authors - Juan Zhang
mouse	IF	J Stroke Cerebrovasc Dis; An Increase of Sigma-1 Receptor in the Penumbra Neuron after Acute Ischemic Stroke.; Authors - Xiaotian Zhang
human	IHC	Pediatr Surg Int; Pathological changes of interstitial cells of Cajal and ganglion cells in the segment of resected bowel in Hirschsprung's disease.; Authors - Xuyong Chen