NeuN Polyklonaler Antikörper

NeuN Polyklonal Antikörper für WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26975-1-PBS

Synonyme

Fox-1 homolog C, FOX3, HRNBP3, NeuN antigen, Neuronal nuclei antigen

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  WB analysis using 26975-1-AP (same clone as 26975-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26975-1-AP (NeuN antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26975-1-PBS in a different storage buffer formulation.

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  WB analysis using 26975-1-AP (same clone as 26975-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26975-1-AP (NeuN antibody) at dilution of 1:80000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26975-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 26975-1-AP (same clone as 26975-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26975-1-AP (NeuN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26975-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 26975-1-AP (same clone as 26975-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 26975-1-AP (NeuN antibody) at dilution of 1:30000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26975-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 26975-1-AP (same clone as 26975-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 26975-1-AP (NeuN antibody) at dilution of 1:30000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26975-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat cerebellum using 26975-1-AP (same clone as 26975-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 26975-1-AP (NeuN antibody) at dilution of 1:30000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26975-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 26975-1-AP (same clone as 26975-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NeuN antibody (26975-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), GFAP antibody (60190-1-Ig, Clone: 4B2E10, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26975-1-PBS in a different storage buffer formulation.

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  IF Staining of rat cerebellum using 26975-1-AP (same clone as 26975-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using 26975-1-AP (NeuN antibody, green), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 60190-1-Ig (GFAP antibody, red). This data was developed using the same antibody clone with 26975-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse cerebellum using 26975-1-AP (same clone as 26975-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NeuN antibody (26975-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26975-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse cerebellum using 26975-1-AP (same clone as 26975-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using NeuN antibody (26975-1-AP) at dilution of 1:5000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26975-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat cerebellum using 26975-1-AP (same clone as 26975-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using 26975-1-AP (NeuN antibody, green), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 60190-1-Ig (GFAP antibody, red). This data was developed using the same antibody clone with 26975-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 26975-1-AP (same clone as 26975-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 26975-1-AP (NeuN antibody), at dilution of 1:100 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 66375-1-Ig (TUBB3 antibody, green). This data was developed using the same antibody clone with 26975-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat cerebellum using 26975-1-AP (same clone as 26975-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using 26975-1-AP (NeuN antibody, green), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 60190-1-Ig (GFAP antibody, red). This data was developed using the same antibody clone with 26975-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 26975-1-AP (same clone as 26975-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using NeuN antibody (26975-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 26975-1-PBS in a different storage buffer formulation.

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  FC experiment of U-87 MG using 26975-1-AP (same clone as 26975-1-PBS)

  1X10^6 U-87 MG cells were intracellularly stained with 0.2 ug Anti-Human NeuN (26975-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 26975-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

26975-1-PBS bindet in WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA NeuN und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NeuN fusion protein Ag25689
• Vollständiger Name: hexaribonucleotide binding protein 3
• Beobachtetes Molekulargewicht: 46-52 kDa
• GenBank-Zugangsnummer: NM_001082575
• Gene symbol: NeuN
• Gene ID (NCBI): 146713
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Function

NeuN, also known as FOX3 or RBFOX3, belongs to a family of tissue-specific splicing regulators and is involved in neural circuitry balance, as well as neurogenesis and synaptogenesis (PMID: 26619789).

Tissue specificity

NeuN is exclusively present in post-mitotic neurons and is absent from neural progenitors, oligodendrocytes, astrocytes, and glia (PMID: 1483388).

Involvement in disease

· NeuN cytoplasmic localization is increased in the neurons of patients with HIV-associated neurocognitive disorders (PMID: 24215932).

Isoforms

There are 4 isoforms of NeuN, migrating in the 45-50 kDa range (PMID: 21747913).

Post-translational modifications

Currently not known.

Cellular localization

NeuN predominantly localizes to the nucleus but can also be present in the cytoplasm. Isoforms of NeuN differ in their cytoplasmic/nucleus localization (PMID: 21747913).