NeuN Monoklonaler Antikörper

NeuN Monoklonal Antikörper für IHC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

3A4C1

Kat-Nr. : 66836-1-PBS

Synonyme

3A4C1, Fox-1 homolog C, FOX3, HRNBP3, NeuN antigen

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  IHC staining of rat cerebellum using 66836-1-Ig (same clone as 66836-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66836-1-Ig (NeuN antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66836-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 66836-1-Ig (same clone as 66836-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66836-1-Ig (NeuN antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66836-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 66836-1-Ig (same clone as 66836-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66836-1-Ig (NeuN antibody) at dilution of 1:20000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66836-1-PBS in a different storage buffer formulation.

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  IF Staining of rat cerebellum using 66836-1-Ig (same clone as 66836-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using 66836-1-Ig (NeuN antibody, red), at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The section was co-stained with 14479-1-AP (Calbindin-D28k antibody, green). This data was developed using the same antibody clone with 66836-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse cerebellum using 66836-1-Ig (same clone as 66836-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using 66836-1-Ig (NeuN antibody), at dilution of 1:100 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The section was co-stained with 14479-1-AP (Calbindin-D28k Antibody, green). This data was developed using the same antibody clone with 66836-1-PBS in a different storage buffer formulation.

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  IF Staining of rat cerebellum using 66836-1-Ig (same clone as 66836-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat cerebellum tissue using NeuN antibody (66836-1-Ig, Clone: 3A4C1 ) at dilution of 1:800 and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8), CoraLite®594 GFAP antibody (CL594-60190, Clone: 4B2E10, red), NF-H/NF200 antibody (18934-1-AP, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66836-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 66836-1-Ig (same clone as 66836-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NeuN antibody (66836-1-Ig, Clone: 3A4C1 ) at dilution of 1:800 and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8), CoraLite® Plus 488 GFAP antibody (CL488-60190, Clone: 4B2E10, green), OLIG2 antibody (13999-1-AP, yellow). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66836-1-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 66836-1-Ig (same clone as 66836-1-PBS)

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human NeuN (66836-1-Ig, Clone:3A4C1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66836-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66836-1-PBS bindet in IHC, IF-P, FC (Intra), ELISA NeuN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NeuN fusion protein Ag28016
• Vollständiger Name: hexaribonucleotide binding protein 3
• GenBank-Zugangsnummer: NM_001082575
• Gene symbol: NeuN
• Gene ID (NCBI): 146713
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NeuN, encoded by FOX3, is a neuron-specific nuclear protein. Anti-NeuN stains exclusively neuronal cells in the central and peripheral nervous systems, especially postmitotic and differentiating neurons, as well as terminally differentiated neurons. Anti-NeuN has been used widely as a reliable tool to detect most postmitotic neuronal cell types. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm.