NeuN Monoklonaler Antikörper

NeuN Monoklonal Antikörper für IF-P, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF-P, FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

CloneNo.

3A4C1

Kat-Nr. : CL555-66836

Synonyme

cDNA FLJ56884, FLJ56884, FLJ58356, FOX3, hCG_1776007, Homo sapiens (Human), HRNBP3, NeuN, RBFOX3

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  IF Staining of rat brain using CL555-66836

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite®555 NeuN antibody (CL555-66836, Clone: 3A4C1 ) at dilution of 1:200.

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  FC experiment of U-87 MG using CL555-66836

  1X10^6 U-87 MG cells were intracellularly stained with 0.8 ug CoraLite®555 Anti-Human NeuN (CL555-66836, Clone:3A4C1) (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Rattenhirngewebe
• Erfolgreiche Detektion in FC (Intra): U-87 MG-Zellen
• Erfolgreiche Detektion in FC: U-87 MG-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
• Durchflusszytometrie (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL555-66836 bindet in IF-P, FC (Intra) NeuN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NeuN fusion protein Ag28016
• Vollständiger Name: hexaribonucleotide binding protein 3
• GenBank-Zugangsnummer: NM_001082575
• Gene symbol: NeuN
• Gene ID (NCBI): 146713
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NeuN, encoded by FOX3, is a neuron-specific nuclear protein. Anti-NeuN stains exclusively neuronal cells in the central and peripheral nervous systems, especially postmitotic and differentiating neurons, as well as terminally differentiated neurons. Anti-NeuN has been used widely as a reliable tool to detect most postmitotic neuronal cell types. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm.