NeuN Monoklonaler Antikörper

NeuN Monoklonal Antikörper für IF-P

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF-P

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

3A4C1

Kat-Nr. : CL594-66836

Synonyme

3A4C1, Fox-1 homolog C, FOX3, HRNBP3, NeuN antigen

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  IF Staining of mouse cerebellum using CL594-66836

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CoraLite®594 NeuN antibody (CL594-66836, Clone: 3A4C1 ) at dilution of 1:200.

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  IF Staining of mouse cerebellum using CL594-66836

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CoraLite®594 NeuN antibody (CL594-66836, Clone: 3A4C1 ) at dilution of 1:200.

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  IF Staining of rat brain using CL594-66836

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using CoraLite®594 NeuN antibody (CL594-66836, Clone: 3A4C1 ) at dilution of 1:400, CoraLite® Plus 647 MAP2 antibody (CL647-84306-3, Clone: 241653E9, Magenta), CoraLite®532 GFAP antibody (CL532-81063, Clone: 4C6, golden), CoraLite® Plus 488 MBP antibody (CL488-10458, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using CL594-66836

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using CoraLite®594 NeuN antibody (CL594-66836, Clone: 3A4C1 ) at dilution of 1:400, CoraLite® Plus 647 MAP2 antibody (CL647-84306-3, Clone: 241653E9, Magenta), CoraLite®532 GFAP antibody (CL532-81063, Clone: 4C6, golden), CoraLite® Plus 488 MBP antibody (CL488-10458, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Maus-Cerebellum-Gewebe, Rattenhirngewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66836 bindet in IF-P NeuN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NeuN fusion protein Ag28016
• Vollständiger Name: hexaribonucleotide binding protein 3
• GenBank-Zugangsnummer: NM_001082575
• Gene symbol: NeuN
• Gene ID (NCBI): 146713
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NeuN, encoded by FOX3, is a neuron-specific nuclear protein. Anti-NeuN stains exclusively neuronal cells in the central and peripheral nervous systems, especially postmitotic and differentiating neurons, as well as terminally differentiated neurons. Anti-NeuN has been used widely as a reliable tool to detect most postmitotic neuronal cell types. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm.