Neudesin/NENF Monoklonaler Antikörper

Neudesin/NENF Monoklonal Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4G9E12

Kat-Nr. : 60131-1-PBS

Synonyme

NENF, Neudesin, 4G9E12, Cell immortalization-related protein 2, Neuron-derived neurotrophic factor

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  WB analysis using 60131-1-Ig (same clone as 60131-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60131-1-Ig (Neudesin/NENF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 60131-1-Ig (same clone as 60131-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 60131-1-Ig (NENF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 60131-1-Ig (same clone as 60131-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum using 60131-1-Ig(NENF antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 60131-1-Ig (same clone as 60131-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum using 60131-1-Ig(NENF antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60131-1-Ig (same clone as 60131-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60131-1-Ig (Neudesin/NENF antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60131-1-Ig (same clone as 60131-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60131-1-Ig (Neudesin/NENF antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 60131-1-Ig (same clone as 60131-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 60131-1-Ig(NENF antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 60131-1-Ig (same clone as 60131-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 60131-1-Ig(NENF antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60131-1-Ig (same clone as 60131-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon slide using 60131-1-Ig (NENF Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 60131-1-Ig (same clone as 60131-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon slide using 60131-1-Ig (NENF Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60131-1-Ig (same clone as 60131-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 60131-1-Ig (NENF Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60131-1-Ig (same clone as 60131-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 60131-1-Ig (NENF Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60131-1-Ig (same clone as 60131-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Neudesin/NENF antibody (60131-1-Ig, Clone: 4G9E12 ) at dilution of 1:1900 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60131-1-Ig (same clone as 60131-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Neudesin/NENF antibody (60131-1-Ig, Clone: 4G9E12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60131-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50685-1

  Cytometric bead array standard curve of MP50685-1, NENF Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60131-1-PBS. Detection antibody: 60131-2-PBS. Standard:Ag8387. Range: 0.098-100 ng/mL.

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  Cytometric bead array standard curve of MP50685-2

  Cytometric bead array standard curve of MP50685-2, NENF Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60131-1-PBS. Detection antibody: 60131-3-PBS. Standard:Ag8387. Range: 0.098-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

60131-1-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Neudesin/NENF und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Neudesin/NENF fusion protein Ag8387
• Vollständiger Name: neuron derived neurotrophic factor
• Berechnetes Molekulargewicht: 172 aa, 19 kDa
• Beobachtetes Molekulargewicht: 19 kDa, 16 kDa
• GenBank-Zugangsnummer: BC008823
• Gene symbol: NENF
• Gene ID (NCBI): 29937
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neudesin neurotrophic factor (NENF, also known as CIR2, SPUF, and SCIRP10) acts as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival, which is localized to mitochondria and endoplasmic reticulum by PINK1 and PARK7 (PMID: 31536960). NENF in the adult brain is expected to play roles in the maintenance and protection of neurons in an autocrine/paracrine manner (PMID: 15605373). It greatly increases cAMP levels in neural precursor cells and might activate a Gs-protein-coupled receptor that could activate the MAPK, PKA, and PI-3K signal pathways (PMID: 16547973).