Neurofibromin 1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Neurofibromin 1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 27249-1-AP

Synonyme

Neurofibromin, NF1, NF 1, NF-1

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  WB analysis using 27249-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27249-1-AP (Neurofibromin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27249-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27249-1-AP (Neurofibromin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 27249-1-AP

  IP result of anti-Neurofibromin 1 (IP:27249-1-AP, 4ug; Detection:27249-1-AP 1:1000) with HEK-293 cells lysate 1800 ug.

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  IHC staining of mouse brain using 27249-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27249-1-AP (Neurofibromin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 27249-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27249-1-AP (Neurofibromin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27249-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27249-1-AP (Neurofibromin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27249-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27249-1-AP (Neurofibromin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 27249-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27249-1-AP (Neurofibromin antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 27249-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27249-1-AP (Neurofibromin antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 27249-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 27249-1-AP (Neurofibromin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HEK-293T-Zellen, HeLa-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Mammakarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

27249-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA Neurofibromin 1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Neurofibromin 1 fusion protein Ag25799
• Vollständiger Name: neurofibromin 1
• Berechnetes Molekulargewicht: 319 kDa
• Beobachtetes Molekulargewicht: 250-280 kDa
• GenBank-Zugangsnummer: M60915
• Gene symbol: Neurofibromin 1
• Gene ID (NCBI): 4763
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NF1 codes for neurofibromin, a GTPase-activating protein that negatively regulates RAS/MAPK pathway activity by accelerating the hydrolysis of Ras-bound GTP. NF1 has a high mutation rate and mutations in NF1 can alter cellular growth control, and neural development, resulting in neurofibromatosis type 1 (NF1, also known as von Recklinghausen syndrome).

Publikationen

Species	Application	Title
		Nat Commun; Targeting NRAS via miR-1304-5p or farnesyltransferase inhibition confers sensitivity to ALK inhibitors in ALK-mutant neuroblastoma; Authors - Perla Pucci
human	WB	Clin Transl Med; IGF2BP3 mediates the mRNA degradation of NF1 to promote triple-negative breast cancer progression via an m6A-dependent manner; Authors - Xu Zhang
human	WB,IHC	Gynecol Oncol; Survey of NF1 inactivation by surrogate immunohistochemistry in ovarian carcinomas; Authors - Martin Köbel
human	WB,IP	Front Genet; Characterization of Two Loss-of-Function NF1 Variants in Chinese Patients and Potential Molecular Interpretations of Phenotypes.; Authors - Tingting Zhang
human	WB	Mol Genet Genomic Med; A novel mutation in NF1 gene of patient with Neurofibromatosis type 1: A case report and functional study.; Authors - Tingting Zhang
human	WB,IHC,IF	J Transl Med; The oncogenic role of NF1 in gallbladder cancer through regulation of YAP1 stability by direct interaction with YAP1; Authors - Lingxiao Zhang ; KD Validated