Neurofibromin Rekombinanter Antikörper

Neurofibromin Rekombinant Antikörper für WB, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240540F2

Kat-Nr. : 83620-5-PBS

Synonyme

NF1, 240540F2, neurofibromin 1, NF 1, NF-1

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  WB analysis using 83620-5-RR (same clone as 83620-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83620-5-RR (Neurofibromin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83620-5-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 83620-5-RR (same clone as 83620-5-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug Neurofibromin 1 Recombinant antibody (83620-5-RR, Clone:240540F2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83620-5-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83620-5-RR (same clone as 83620-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Neurofibromin 1 Recombinant antibody (83620-5-RR, Clone:240540F2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83620-5-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00570-2

  Cytometric bead array standard curve of MP00570-2, Neurofibromin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83620-5-PBS. Detection antibody: 83620-4-PBS. Standard: Ag25799. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 83620-5-PBS

  Biolayer interferometry (BLl) kinetic assay of 83620-5-PBS against Human Neurofibromin 1 was performed. The affinity constant is 68.2 pM.

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  Affinity and Kinetic Characterization of 83620-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83620-5-RR against Human Neurofibromin 1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83620-5-PBS bindet in WB, FC (Intra), Cytometric bead array, Indirect ELISA Neurofibromin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Neurofibromin fusion protein Ag25799
• Vollständiger Name: neurofibromin 1
• Berechnetes Molekulargewicht: 319 kDa
• Beobachtetes Molekulargewicht: 250-280 kDa
• GenBank-Zugangsnummer: M60915
• Gene symbol: Neurofibromin 1
• Gene ID (NCBI): 4763
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NF1 codes for neurofibromin, a GTPase-activating protein that negatively regulates RAS/MAPK pathway activity by accelerating the hydrolysis of Ras-bound GTP. NF1 has a high mutation rate and mutations in NF1 can alter cellular growth control, and neural development, resulting in neurofibromatosis type 1 (NF1, also known as von Recklinghausen syndrome).