Neuropilin 1/CD304 Rekombinanter Antikörper

Neuropilin 1/CD304 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241812G11

Kat-Nr. : 84429-5-PBS

Synonyme

neuropilin 1, NRP1, 241812G11, BDCA4, CD304

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  WB analysis using 84429-5-RR (same clone as 84429-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84429-5-RR (NRP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84429-5-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84429-5-RR (same clone as 84429-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84429-5-RR (Neuropilin 1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84429-5-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84429-5-RR (same clone as 84429-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84429-5-RR (Neuropilin 1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84429-5-PBS in a different storage buffer formulation.

• 

  IF Staining of PC-3 using 84429-5-RR (same clone as 84429-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using Neuropilin 1 antibody (84429-5-RR, Clone: 241812G11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84429-5-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84429-5-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Neuropilin 1/CD304 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Neuropilin 1/CD304 fusion protein Eg2331
• Vollständiger Name: neuropilin 1
• Beobachtetes Molekulargewicht: 130-140 kDa
• GenBank-Zugangsnummer: BC007533
• Gene symbol: Neuropilin 1
• Gene ID (NCBI): 8829
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neuropilin-1 (NRP1) is a 130-140 kDa transmembrane glycoprotein expressed by endothelial, dendritic, and regulatory T cells, as well as several other normal cell types and malignant tumor cells. NRP1 was first identified as a semaphorin (SEMA) receptor, involved in axonal guidance in embryonic development. NRP1 was also shown to act as a receptor for vascular endothelial growth factor (VEGF) and a promoter of angiogenesis through its interaction with VEGF-A165 (and other VEGFs) and the receptor tyrosine kinase (RTK) VEGF-R2. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.