Neuroserpin Monoklonaler Antikörper

Neuroserpin Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G4C6

Kat-Nr. : 66997-1-PBS

Synonyme

Serpin I1, 1G4C6, Peptidase inhibitor 12, PI 12, PI12

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  WB analysis of pig brain using 66997-1-Ig (same clone as 66997-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66997-1-Ig (SERPINI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66997-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 66997-1-Ig (same clone as 66997-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66997-1-Ig (Neuroserpin antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66997-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 66997-1-Ig (same clone as 66997-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66997-1-Ig (Neuroserpin antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66997-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 66997-1-Ig (same clone as 66997-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using Neuroserpin antibody (66997-1-Ig, Clone: 1G4C6 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66997-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66997-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Neuroserpin und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Neuroserpin fusion protein Ag28636
• Vollständiger Name: serpin peptidase inhibitor, clade I (neuroserpin), member 1
• Berechnetes Molekulargewicht: 410 aa, 47 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC018043
• Gene symbol: Neuroserpin
• Gene ID (NCBI): 5274
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neuroserpin is a member of the serpin family of serine protease inhibitors predominantly expressed in the nervous system, such as s the cerebral cortex, hippocampus and the spinal cord.Neuroserpin exerts protective effects in neurons against excitotoxicity both in vitro and in vivo. Its proteolytic inhibitory activity is shown to protect neurons against damage caused by plasmin activation. Mice overexpressing neuroserpin exhibited loss of tPA activity in the brain. Overexpression of neuroserpin in primary neurons leads to increased dendritic arborization and altered dendritic spine shape.