Neuroserpin Rekombinanter Antikörper

Neuroserpin Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250112E7

Kat-Nr. : 85923-1-PBS

Synonyme

Serpin I1, Peptidase inhibitor 12, PI 12, PI12, PI-12

---

• 

  WB analysis using 85923-1-RR (same clone as 85923-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85923-1-RR (SERPINI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85923-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 85923-1-RR (same clone as 85923-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85923-1-RR (Neuroserpin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85923-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 85923-1-RR (same clone as 85923-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85923-1-RR (SERPINI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85923-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 85923-1-RR (same clone as 85923-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85923-1-RR (Neuroserpin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85923-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

85923-1-PBS bindet in WB, IHC, Indirect ELISA Neuroserpin und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Neuroserpin fusion protein Eg2819
• Vollständiger Name: serpin peptidase inhibitor, clade I (neuroserpin), member 1
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: NM_005025.4
• Gene symbol: Neuroserpin
• Gene ID (NCBI): 5274
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neuroserpin is a member of the serpin family of serine protease inhibitors predominantly expressed in the nervous system, such as s the cerebral cortex, hippocampus and the spinal cord.Neuroserpin exerts protective effects in neurons against excitotoxicity both in vitro and in vivo. Its proteolytic inhibitory activity is shown to protect neurons against damage caused by plasmin activation. Mice overexpressing neuroserpin exhibited loss of tPA activity in the brain. Overexpression of neuroserpin in primary neurons leads to increased dendritic arborization and altered dendritic spine shape.