Neuroserpin Rekombinanter Antikörper

Neuroserpin Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250112E7

Kat-Nr. : 85923-1-RR

Synonyme

Serpin I1, Peptidase inhibitor 12, PI 12, PI12, PI-12

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  WB analysis using 85923-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85923-1-RR (SERPINI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85923-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85923-1-RR (Neuroserpin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85923-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85923-1-RR (SERPINI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 85923-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85923-1-RR (Neuroserpin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, Maushirngewebe, Maus-Cerebellum-Gewebe, Hausschwein-Hirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85923-1-RR bindet in WB, IHC, ELISA Neuroserpin und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Neuroserpin fusion protein Eg2819
• Vollständiger Name: serpin peptidase inhibitor, clade I (neuroserpin), member 1
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: NM_005025.4
• Gene symbol: Neuroserpin
• Gene ID (NCBI): 5274
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neuroserpin is a member of the serpin family of serine protease inhibitors predominantly expressed in the nervous system, such as s the cerebral cortex, hippocampus and the spinal cord.Neuroserpin exerts protective effects in neurons against excitotoxicity both in vitro and in vivo. Its proteolytic inhibitory activity is shown to protect neurons against damage caused by plasmin activation. Mice overexpressing neuroserpin exhibited loss of tPA activity in the brain. Overexpression of neuroserpin in primary neurons leads to increased dendritic arborization and altered dendritic spine shape.