NeutraKine®FGF basic Monoklonaler Antikörper

NeutraKine®FGF basic Monoklonal Antikörper für Neutralization, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

Neutralization, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3C9C3

Kat-Nr. : 69024-1-PBS

Synonyme

FGF2, FGF-2, 3C9C3, FGF 2, FGF B

---

• 

  Neutralization experiment of NeutraKine®FGF basic using 69024-1-Ig (same clone as 69024-1-PBS)

  Recombinant human FGF Basic (Cat.NO. HZ-1285) stimulates the proliferation of BALB/3T3 cells (mouse fibroblast cell) at low FBS condition in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human FGF Basic (0.1 ng/mL HZ-1285) is neutralized by mouse anti-human FGF Basic monoclonal antibody 69024-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 1-10 μg/mL. Mouse IgG1 Isotype Control monoclonal antibody 66360-1-Ig was served as control (green curve, refer to top X-right Y axis). This data was developed using the same antibody clone with 69024-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50262-2

  Cytometric bead array standard curve of MP50262-2, FGF2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68870-2-PBS. Detection antibody: 69024-1-PBS. Standard:HZ-1285. Range: 0.391-100 ng/mL.

---

Produktinformation

69024-1-PBS bindet in Neutralization, Cytometric bead array, Indirect ELISA NeutraKine®FGF basic und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: human HumankineFGF basic protein HZ-1285
• Vollständiger Name: fibroblast growth factor 2 (basic)
• Gene symbol: FGF2
• Gene ID (NCBI): 2247
• Konjugation: Unkonjugiert
• Form: Lyophilized Powder
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Endotoxin: <0.1 EU/μg
• Wiederherstellung: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Hintergrundinformationen

Fibroblast growth factor-2 (FGF-2), also referred to as basic FGF, belongs to a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in at least five different isoforms with distinct properties.

This antibody is used to neutralize the bioactivity of FGFbasic-TS.