Nexilin Polyklonaler Antikörper

Nexilin Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27737-1-PBS

Synonyme

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  WB analysis using 27737-1-AP (same clone as 27737-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27737-1-AP (Nexilin antibody) at dilution of 1:80000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27737-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 27737-1-AP (same clone as 27737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27737-1-AP (Nexilin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27737-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 27737-1-AP (same clone as 27737-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27737-1-AP (Nexilin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27737-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 27737-1-AP (same clone as 27737-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Nexilin antibody (27737-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27737-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 27737-1-AP (same clone as 27737-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Nexilin antibody (27737-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27737-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27737-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA Nexilin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Nexilin fusion protein Ag26938
• Vollständiger Name: nexilin (F actin binding protein)
• Berechnetes Molekulargewicht: 675 aa, 81 kDa
• Beobachtetes Molekulargewicht: 80-85 kDa
• GenBank-Zugangsnummer: BC114444
• Gene symbol: Nexilin
• Gene ID (NCBI): 91624
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nexilin, Also known as NEXN, NELIN, is a filamentous actin-binding protein that localizes to focal contacts and may be involved in cell adhesion and migration(PMID: 15823560). Nexilin is abundantly expressed in heart and skeletal muscle and has an essential role in the maintenance of Z line and sarcomere integrity(PMID: 12053183, 19881492). Mutations in Nexilin have been associated with cardiomyopathy dilated type 1CC (CMD1CC) and cardiomyopathy familial hypertrophic type 20 (CMH20)(PMID: 19881492, 20970104).